cd11a cd18 fitc  (Miltenyi Biotec)

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Identification of a Gene Expression Signature to Predict the Risk of Abdominal Aortic Aneurysm in Psoriasis Patients

doi: 10.2147/CCID.S495890

Figure Lengend Snippet: Establishment of gene signature by LASSO regression. ( A and B ) Heatmap of 20 hub genes in GSE226244 ( A ) and GSE57691 ( B ). Gene expression is scaled by the Z-score. The bar plot beside the heatmap reveals the log2FC of genes. ( C ) The PPI network constructed by STRING reveals the interaction between these 20 genes. ( D ) Friend analysis discovers the rank of 20 genes. The x-axis indicates the gene similarity. The y-axis represents genes. ( E ) The log (lambda) sequence was used to construct a coefficient profile diagram. ( F ) LASSO coefficient profiles of the 20 genes in psoriasis. ( G and H ) ROC curve of CCR7, CD3D, GBP5, HCLS1, IL7R, ITGAL, and the gene signature. ( I ) The visible nomogram for diagnosing psoriasis.

Article Snippet: We performed immunohistochemical (IHC) analysis to assess the differential expressions of CCR7 (1:100 dilution, Proteintech,55425-1-AP, Wuhan, China); CD3D (1:200 dilution, Proteintech,16669-1-lg, Wuhan, China); GBP5 (1:200 dilution, Proteintech,13220-1-AP, Wuhan, China); HCLS1 (1:200 dilution Proteintech,25003-1-lg, Wuhan, China); IL7R (1:100 dilution, Abcam, ab259806, Cambridge, UK), and ITGAL (1:100 dilution, Proteintech,15574-1-AP, Wuhan, China) in different groups.

Techniques: Gene Expression, Construct, Sequencing